摘要
本文首次应用地鼠血清培养异合卵制备人精子染色体标本，获得显著的效果。同时应用胎牛血精进行了比较，在两种血清中精子染色体形成率分别为１０２．４５％和５８．２８％，可供分析核型获得率分别为７６．１５％和３１．５１％，两组间存在非常显著的差异（Ｐ＜０．００１）．观察分析了６例供精者的４９８个精子核型，数目异常和结构异常发生率分别为０．８％和２．８％。本研究提示地鼠血清培养同种属卵，可促进卵细胞在体外的进一步成熟，并有助于人精原核的发育及其染色体的形成。
This study reporr a method for preparating human sperm chromosome with ham-ster serum for postinsemination culture of the zona-free hamster ova.The rate of analyzablekaryotype was markedly increased.Comparison hamster serum with fetal bovine serum.Theaverage rate to reach the first-cleavage was 102.45%in the inseminated oocytes versus 58.28%.The rate of observable karyotyping in two sera were 76.15%versus 31.51%(P<0.001).Spontaneous incidence of sperm chromosome aberrations was analyzed in a total of 498spermatozoa obtained from six donors.Incidence of aneuploidy and structural abnormalitywere 0.8%(hypohaploidy)and 2.81%,respectively.This result suggest that the hamsterserum may be more suitable to the further maturity of hamster ova in vitro and contribute tothe growth and development of male pronuclear chromosome.
出处
《癌变．畸变．突变》
CAS
CSCD
1996年第2期117-121,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
金黄地鼠血清
异合卵培养
人精子染色体
Hamster serum
Postinsemination cultrue
Human sperm chromosome
