摘要
用恶性疟原虫主要裂殖子表面抗原基因序列（ＭＳＡ－１）为引物的聚合酶链反应（ＰＣＲ）技术检测恶性疟原虫感染。用此方法扩增实验室体外培养的ＦＣＣ１／ＨＮ株恶性疟原虫，显示１条３００ｂｐ的ＤＮＡ片断，而对实验室培养的巴布亚新几内亚ＦＣＱ－２７株则显示１条４４０ｂｐＤＮＡ条带。用此方法检测１５份采自云南中缅边界的恶性疟血样均能扩增出ＤＮＡ条带，但不同血样的ＤＮＡ条带的分子量不同，部分血样还存在１条以上的ＤＮＡ条带。在同时扩增的１０份正常人血样和１０份采自江苏间日疟流行区的现症间日疟血样中均没有发现ＤＮＡ条带。表明此方法具有很高的种和株的特异性。
Polymerase Chain Reaction(PCR) using a pair of P.falciparum specific primers(PfMSA-1) was used to detect P. falciparum infection.The results showed that DNA fragment of 300bp was amplified in cultured P.falciparum isolate FCC1/HN and a DNA fragment of 440bp in cultured P. falciparum isolate FCQ-27/PNG.This technique has been used to detect blood samples collected form Yunnan endemic areas.All of the 15 microscopically confirmed P.falciparum blood samples were amplified DNA fragments,but the molecular weight of amplified DNA fragments were different in different P.falciparum cases(440bp,310bp,300bp,280bp, 270bp and 110bp).At the same time, no DNA fragments were amplified in 10 P.vivax samples collected from Jiangsu P.vivaxendemic area and 10 normal blood samples collected from Jiangsu non-endemic area.
出处
《中国寄生虫病防治杂志》
CSCD
1996年第1期10-12,共3页
Chinese Journal of Parasitic Disease Control
基金
联合国开发计划署/世界银行/世界卫生组织热带病研究和培训特别规划处的资助
