摘要
通过模拟低压低氧(7000±50 m)考察谷胱苷肽转移酶p1基因(G lu tath ione S-transferase p1,G STP 1)对低压低氧的敏感性,以探讨G STP 1在机体适应急性低压低氧环境中的分子机制。30只雄性SD大鼠随机分为5组:0、1、3、5、7 d组,每天在模拟7000±50 m高度的低压低氧舱内暴露12 h,连续暴露6 h后,休息1 h,再继续暴露6h。采用荧光定量-反转录-聚合酶链反应(FQ-RT-PCR)技术对急性低压低氧大鼠模型肺组织中G STP 1基因表达进行定量分析,同时用分光光度法测定了肺组织中谷胱苷肽转移酶(g lu tath ione S-transferases,G ST s)的活性和丙二醛(M a le ic d ia ldehyde,M DA)的变化。结果显示,与未暴露组相比,G STP 1基因从第1 d到第7 d的表达有显著性差异(P<0.05);G ST s活性从第1 d到第7 d呈下降趋势,M DA呈上升趋势,与未暴露组相比,均有显著性差异(P<0.05)。结果表明:G STP 1基因对低压低氧敏感,并可考虑作为机体适应特殊环境进行基因选材的一个新指标。
We have investigated the susceptibility of rat lung＇s GSTP1 gene to hypobarie hypoxia and explored its role in the body＇s possible adaptation mechanism at the moleuelar lever. Thirty male SD rats were randomly divided into five groups （0,1,3,5 and 7 d） and were exposed for 12 h per day at a simulating altitude of 7000± 50 m in a hypoharie hypoxia chamber with 1 h＇s rest after 6 h＇s exposure. Then the expression of GSTP1 mRNA in the lung tissue of SD rats was examined using fluorescence quantitative RT-PCR. Meanwhile the activity of glutathione S-transferases （GSTs） enzyme and the change of maleie dialdehyde （MDA） in the lung tissue of SD rats were determined using speetrophotometer. In comparison with the non-exposure group,the expression of GSTP1 gene showed statistically significant differnee from the first to the seventh day（P〈0.05）. The level of GSTs decreased and MDA increased from the first to the seventh day （P〈0.05）. In conclusion, GSTP1 gene is susceptible to hypobarie hypoxia and may be a new marker of gene screening for the body＇s adaptation to special environment.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2006年第2期405-409,共5页
Journal of Biomedical Engineering
关键词
胱苷肽转移酶p1基因
低压低氧
荧光定量反转录聚合酶链反应
Glutathione S-transferase p1 （GSTP1） Hypobaric hypoxia Fluorescence quantitative reverse transcription-coupled polymerase chain reaction（FQ-RT-PCR）
