摘要
研究生物材料对血小板信号转导关键成分——黏着斑激酶(FAK)磷酸化的影响。采用PVC、PU 50、PU 60、PU 70和PTFE管与比格犬股动静脉建立半体内循环,循环时间为5 m in,运用免疫沉淀和蛋白印迹方法检测管内壁黏附血小板的磷酸化FAK含量和FAK含量。结果表明:PVC和PTFE的FAK含量明显高于三种聚氨酯材料,三种聚氨酯材料中以PU 70最高,PU 60次之,PU 50最低。五种材料的磷酸化FAK含量以PVC最高;PTFE次之;PU 70、PU 60和PU 50最低,三种聚氨酯材料之间无明显的差异。由此提示:生物材料与血小板接触后,可能会引起血小板信号转导系统的关键酶FAK发生磷酸化,材料的血液相容性越差,FAK磷酸化越明显;磷酸化FAK含量可考虑作为在分子水平上评价材料血液相容性优劣的有效指标;FAK含量可为材料相容性评价提供参考作用。
This study was aimed to detect the biomaterial-induced phosphorylation level of platelet focal adhesion kinase, a key component of signal transduction pathways. Flat tubings, made from 5 biomaterials： organotin-PVC, PU50, PU60, PU70, and PTFE,were connected to an ex vivo flow circuit with canine femoral artery and vein. After 5 min, the adherent platelets on the inner surface of the tubing were lysed, then FAK and phospho-FAK were examined, using immunoprecipitation and Western blot. The results showed a significant increase in the FAK value on the PVC and PTFE surface,compared to the PUs; among the PUs, the FAK value decreased in the following order： PU70/PU60/PU50. The phospho-FAK value on the five groups of surface decreased in the following order： PVC/PTFE/PU70, PU60, PU50; there was no significant difference among the PUs with regard to the phospho-FAK value. In conclusion, after platelet-material interaction, biomateriais may lead to the phosphorylation of FAK of platelets;the level of phosphorylation of FAK has been positively linked to the thrombogenicity of materials; the phospho-FAK value may be a valid molecular parameter of the haemocompatibility assessment ; the FAK value may supply references to the haemocompatibility assessment.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2006年第2期350-352,共3页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(30270390)
关键词
生物材料
血液相容性
血小板
黏着斑激酶
磷酸化
Biomaterials Hemoeompatibility Platelet Focal adhesion kinase Phosphorylation
