摘要
目的:为了比较胃癌高、低发区幽门螺杆菌(helibobactorpylori,Hp)毒力基因菌株感染的差异,进而揭示Hp菌株分布的地域性特征,探讨Hp感染的流行病学规律及其疾病相关性。方法:选取胃黏膜活检标本491例,其中庄河地区355例,沈阳地区136例。在微需氧的条件下,进行Hp培养,并用标准的酚-氯仿方法提取菌种DNA后,经聚合酶链反应及琼脂糖凝胶电泳对cagA、vacA和iceA基因亚型进行检测。同时取胃窦、体和角黏膜各一块,经石蜡包埋,HE染色,行组织病理学诊断。结果:vacAs1基因亚型在胃癌高发区的检出率为95·33%,与低发区的64·29%相比差异有统计学意义,χ2=25·008,P=0·000。在高发区感染vacAs1m1b型菌株有22例(20·56%),沈阳地区仅1例(2·38%),两者差异有统计学意义,χ2=7·637,P=0·006。结论:辽宁省庄河、沈阳两地Hp菌株基因型的分布存在明显差异,感染高毒力型vacAs1或vacAs1m1菌株在庄河地区明显高于沈阳地区,此点可能是高发区胃癌发病率高的危险因素之一,对这种菌株的研究,可以为揭示Hp的致病机制提供线索。
OBJECTIVE：To compare the distribution of virulence-associated genotypes of Helicobacter. pylori in two Chinese populations with contrasting gastric cancer risk and furthermore probe into the pathogenicity of the bacterium.METHODS： Gastric biopsies gastric biopsies were taken from 355 subjects from Zhuanghe, the high risk area of gastric cancer, and 136 subjects from Shenyang, the low risk area of China. A total of 149H. pylori strains isolated from these patients were studied by PCR for detecting the genotypes of cagA, vacA, and iceA. RESULTS： In patients from the population with high risk for gastric cancer, statistically significant higher relative frequencies of vacA s1 （95. 33% vs 64. 29%, x^2 = 25. 008,P=0. 000） or s1mlb （20. 56% vs 2. 38%, x^2=7. 637,P= 0. 006） genotypes were found as compared to the population from the low risk area. CONCLUSION： There was significant difference distribution of H. pylori genotypes between Zhuanghe area and Shenyang area. The presence of vacA sl or vacA slmlb strain in Zhuanghe is higher than that in Shenyang. Tt is suggested that virulence associated genes of H. pylori may be a factor contributed to the higher gastric cancer incidence rate in the high-risk area compared with the low. In the future, it is necessary to make deeply research at this strain. It will give a clue to make sure the bacterial mecbanisarn.
出处
《中华肿瘤防治杂志》
CAS
2006年第4期252-256,共5页
Chinese Journal of Cancer Prevention and Treatment
基金
国家"十五"科技攻关项目[2001BA703B06(B)]
关键词
胃肿瘤/流行病学
螺杆菌
幽门/遗传学
螺杆菌感染
基因
细菌
细菌蛋白质类
stomach neoplasms/epidemiology
helicobacter pylori/genetics
helicobacter infections
genes, bacterial
bacterial protenis
