摘要
冰冻干燥方法是保存血细胞最佳的方法,因为冻干的血细胞制品能在常温下保存,性能稳定,保存时间长,便于运输,保存费用低廉等优势,解决了目前血细胞保存的局限性。然而,在冻干过程中血细胞膜的损伤、细胞功能的降低是一个重要的问题。目前,研究者多以海藻糖为保护剂,将海藻糖导入血细胞内,对血细胞进行冻干。本文主要综述冻干对血细胞损伤机理,海藻糖对血细胞冻干过程中的保护机制及海藻糖在血细胞冻干保存中的研究现状。
Lyophilization is the best method for preservation of blood cells at present. Lyophilized blood cells could be stored at room temperature for long periods of time, while maintaining a high degree of viability. Lyophilized blood cells facilitates transportation and the costs are low. However, the membrane of blood cells is damaged and viability of blood cells is decressed in lyophilization. Trehalose has been shown to protect membrane, proteins and nucleic acids during freezing and desiccation. Now, researchers present a methor for loading blood cells with trehalose. In this paper, damage effect of lyophilization on blood cells, the mechanism of trehalose protection and the experimantai studies on trelialose were reviewed.
出处
《中国实验血液学杂志》
CAS
CSCD
2006年第2期416-418,共3页
Journal of Experimental Hematology
关键词
海藻糖
血细胞
冻干保存
trehalose
blood cells
lyophilization
