摘要
从福寿螺的胃部组织中获得总RNA,经RT-PCR扩增、测序得到1条新糖苷水解酶基因片段。分析显示其ORF(Open Reading Frame)全长882 bp,编码294个氨基酸。与福寿螺多功能纤维素酶EGX的C端高度同源性达到89.8%,含有糖苷水解酶第10家族的保守性氨基酸序列。
As a mollusk degrading the plant biomass rapidly, Arnpullaria crossean can produce cellulose-hydrolytic enzymes. This brings us a new sight into the biological degradation of the plant biomass. Total RNA was isolated from the stomach of the mollusk Arnpullaria crossean by using polymerase chain reaction, cloning and sequencing techniques, a complementary DNA DY1300 encoding a low molecular mass glycosyl hydrolase has been identified. It contains a 882 nucleotide ORF encoding a 294 amino-acid protein. The percentage of similarity with the cellulase EGX gene sequence from Arnpullaria crossean is 89.8%. Amino acid sequence-based classification has revealed that the putative protein belongs to the glycosyl hydrolase family 10（GHF10）.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第2期29-32,共4页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家重点基础性研究"973"项目(2003CB7160004)
国家自然科学重点基金资助项目(20336010)
关键词
福寿螺
糖苷水解酶
纤维素
木聚糖
Ampullaria crossean
Glycosyl hydrolase
Cellulose
Xylan
