摘要
比较了几种霉菌(毛霉、根霉、曲霉和青霉)在对数生长末期和稳定期末期的胞内和胞外甲壳素脱乙酰酶的活力。研究结果表明:(1)各霉菌的胞内和胞外都具有甲壳素脱乙酰酶活力,而且胞外酶的活力普遍大于胞内酶的活力;(2)处于对数生长期末期的毛霉菌株产甲壳素脱乙酰酶活力最高,其胞外酶活力达到了97.2±4.2 U/g干菌丝体。酶学特性研究表明:该酶的最适温度为50℃,最适pH约为7.2,低温保存稳定性较差,每24小时酶活力下降30%以上。
Activities of intracellular and intercellular chitin deacetylase were compared produced from several mold strains growing at the end of log phase and stable phase respectively. The experimental results indicated that （ 1 ） the activities of chitin deacetylase were found inside and outside the cells, and intercellular enzyme was more active than the intracellular one. And （2）the intercellular enzyme growing at the end of log phase had the highest activity, up to 97.2 ± 4.2 U/g dry mycelia, among mold strains. The study of the enzyme properties showed that the optimum temperature and pH were 50 ℃ and 7.2 respectively. It was also found that the enzyme had a less stability at 4 ℃ and the decrease in the enzyme activity per 24 hours was more than 30 %.
出处
《上海水产大学学报》
CSCD
北大核心
2006年第2期211-215,共5页
Journal of Shanghai Fisheries University
基金
上海水产大学青年基金资助项目(01-48)
