摘要
用醇提-酸醇水解法提取了龙葵内的澳洲茄胺,对其进行了定性、定量分析;以澳洲茄胺为参照品,表征了甾体类生物碱在龙葵中所占质量分数,考察了龙葵果实成长期内甾体类生物碱的形成、变化趋势。发现甾体类生物碱主要富集在龙葵未成熟果实中,检测出其在未成熟果实内的质量分数最高达到3.52%;茎叶所含甾体类生物碱的质量分数低于0.5%。果实内甾体类生物碱的变化在其生长中经历了增加、减少两个时段,在结果一个月后,果实内的甾体类生物碱质量分数达到最大,高达2.5%以上;果实成熟时甾体类生物碱质量分数反而最小,低于1%;阳光照射程度的不同会改变龙葵果实的生长期,从而对甾体类生物碱的质量分数变化产生影响。因此,龙葵的采摘及其生物碱入药,需着重考虑生物碱在该植物不同部位的分布及生长周期、阳光照射等因素。
Solasodine was extracted by the method of ethanol extraction - acid and ethanol hydrolysis, then analyzed qualitively and quantitatively by HPLC equipped with mass detector and UV detector. By detecting solasodine, the content of steroidal alkaloid and its formation and variation in the fruit of Solalum Nigrum L. during the growth is learned. Analyses ＂of the immature fruit, stem and leaf of Solalum Nigrum L. confirmed that steroidal alkaloid was most dominant [ w （steroidal alkaloid） = 3.52% ] in its immature fruit and only minor in its stem and leaf（ mass percent less than 0. 5% ）. The content of steroidal alkaloid in the fruit of Solalum Nigrum L. changes with its growth circle. One month after the fruit come into being, the mass percent of steroidal alkaloid arrived the peak value, then it decreased to minimum once the fruit is completely mature. The time of growth and sunlight are important factor for the formation of steroidal alkaloid in the fruit. So, by learning the content and variation of steroidal alkaloid, the best time of picking the fruit of Solalum Nigrum L. can be made sure. Factors such as the position where alkaloid is enriched, period of growth and sunlight should be considered when steroidal alkaloid derived from Solalum Nigrum L. is used as medicinal materials.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2006年第4期358-361,共4页
Fine Chemicals
