摘要
目的在自来水中痕量有机污染物检测基础上,用微核试验研究其对人体健康的潜在危害。方法采集某市3个自来水厂出厂水(A、B、C)和对照水样(D),XAD-2树脂富集自来水样中的痕量有机物,采用气相色谱质谱(GC/MS)联用法检测分析;小鼠骨髓嗜多染红细胞微核试验检测致突变性。结果在选定条件下,本次试验累计检出痕量有机物335种,其中美国环保局(EPA)优先污染物10余种,致癌、致畸、致突变物20余种。A水厂出厂水小鼠骨髓嗜多染红细胞微核试验中微核率升高(P<0.01),存在剂量-反应关系。B、C水厂出厂水、对照水样小鼠骨髓嗜多染红细胞为阴性。结论GC/MS联用分析和小鼠骨髓嗜多染红细胞微粒试验联合运用是研究饮水中有机污染物致突变性的有效方法。
Objective To study the main organic pollutants in tap water and their genotoxicity in Kunming city. Methods One hundred and fifty liters tap water sample were collected from three water works（A, B, C）and a control group（D）. The organic pollutants in tap water were extracted by XAD - 2 resin, then washed by methanol and acetone. The organic extractions were detected by using Gas Chromatography/Mass Spectrometry（GC/MS） ;Mouse micronucleus test（ MT）was used to detect the genotoxicity effect of organic pollutants in vivo. Results Total 335 organic pollutants were monitored under a certain condition in the above - mentioned water samples, including up to ten kinds of Environmental Protection Agency （EPA） priory pollutants and up to 20 kinds of mutagens and carcinogens. The micronucleus test results demonstrated that organic extraction from waterworks A could induce increasing micronucleus frequency and did not show dose response relationship. Organic extraction from waterworks B and C could not induce increasing micronucleus frequency and did not show dose response relationship. The micronucleus test results of control sample were negative, too. Conclusion The combination of GC/MS and micronucleus test is a rapid and effective method for evaluating organic pollutants in tap water.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2006年第4期476-477,共2页
Chinese Journal of Public Health
基金
云南省应用基础研究基金资助项目(98C061M)
关键词
自来水
有机污染物
GC/MS
微核试验
致突变性
tap water
organic pollutants
Gas Chromatography/Mass Spectrometry（GC/MS）
micronucleus test
Mutagenicity
