摘要
通过ＤＥＡＥ－ＣｅｌｌｕｌｏｓｅＤＥ－５２和ＤＥＡＥ－ＳｅＰｈａｄｅｘＡ－５０离子交换层析等提纯步骤，对解脂假丝酵母胞外脂肪酶进行了纯化，得到了层析纯样品，比活提高２７．４倍，得率１１％．该酶反应最适温度为４０℃；最适ＰＨ为７．５；等电点约在ＰＨ４．５～５．０之间；８℃以下存放，酶活力损失较少，３０℃以上存放，酶活力有明显损失．金属离子Ｓｒ２＋，Ｂａ２＋，Ｚｎ２＋和Ｍｇ２＋对该酶的激活作用为Ｓｒ２＋＞Ｂａ２＋＞Ｚｎ２＋＞Ｍｇ２＋．Ｃｕ２＋和Ｎａ＋对酶活性有抑制作用．ＮａＮ３对保持酶活性有重要作用．
The extracellular lipase from candida lipolytica was purified by means of ion exchange chromatography on DEAE-cellulose DE-52 and DEAE-Sephadex A-50. It was shown that the optimum PH and temperature for the activity of the purified enzyme were at 7. 5 and 40℃, respectively. The enzyme activity was stimulated with various metal ions, the order of the activation is Sr2+>Ba2+>Zn2+>Mg2+, but inhibited by Cu2+ and Na+. NaN3 had remarkable effect on retaining the enzyme activity.The purified enzyme was stable below 8℃. Approximately 6% of the activity was lost after the enzyme was exposed at 16℃ for 24 h. The loss of the activity was serious above 30℃. The isoelectric point was in the range of PH 4. 5-5. 0.
出处
《吉林大学自然科学学报》
CSCD
1996年第3期91-95,共5页
Acta Scientiarum Naturalium Universitatis Jilinensis
关键词
解脂假丝酵母
脂肪酶
提纯
酶制剂
candida lipolytica, lipase, purification, properity
