摘要
采用酶联免疫吸附实验(ELISA)和荧光定量PCR的方法检测壳聚糖碘液对血清HBsAg和HBV DNA的破坏作用。实验结果表明1.56250 g/L和0.78125 g/L的壳聚糖碘液分别作用5 m in和10 m in能完全破坏HBsAg的抗原性;6.25000 g/L和3.12500 g/L的壳聚糖碘液分别作用5 m in和15 m in能较好地破坏血清中的HBV DNA。提示壳聚糖碘液具有体外灭活乙型肝炎病毒作用。
By ELISA and fluorescence quantitative PCR （ FQ - PCR）, the destructive effect of chitson -I2 against the HBsAg and HBV DNA were investigated. The results showed Chitson -I2 solution of 1. 56250 g/L（5 min） 和0.78125 g/L（ 10 min） could destruct antigenicity of serum HBsAg. Chitson - I2 solution of 6.25000 g/L（5 min）和3. 12500 g/L（ 15 min） had a good destructive effect on HBV DNA. Suggesting Chitson- 12 solution had a good destructive effect on Hepatitis B vires in vitro.
出处
《湖南环境生物职业技术学院学报》
CAS
2006年第1期5-7,共3页
JOurnal of Hunan Environment Biological Polytechnic
