摘要
目的：测定参茸口服液人参皂苷Rg1的含量。方法：用高效液相色谱法测定Rg1的含量。色谱分析采用Diamonsil^TM（钻石），C18色谱分析柱，流动相为乙腈磷酸二氢钾缓冲液（25：75）pH3．5，柱温30℃；紫外检测波长为203nm流速为1．0mL·min^-1。实验数据用Gilson 715液相色谱工作站处理。以峰面积外标法定量。结果：人参皂苷Rg1进样量在0．10042～0．90378μg，与峰面积分值呈良好的线性关系。r=0．9996（n=5），平均回收率为100．06％。结论：此方法简便、快速、精度高、分离度好。可作为参茸口服液成品中人参皂苷Rg1的定量分析。
OBJECTIVE To determine ginsenoside Rg1 in Renax oral liquids. METHODS Rg1 was tested by HPLC using C18 chromfographic column with aeetonifrile-0. 05% phosphoric acid（25：75） as mobile phase. The column temperature was 30℃ ,the wavelength for detection was 203 nm, flow rate was 1.0 mL·min^-1. The experiment data were treated with Gilson 715 chromatographic workstation and calculated by peak area and external standard method. RESULTS The recovery of schizandrin was 100.06%,RSD was 1.14%. CONCLUSION The method is found to be convenient,sensitive and accurate.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2006年第4期433-435,共3页
Chinese Journal of Hospital Pharmacy
关键词
人参皂苷RG1
高效液相色谱法
参茸口服液
ginsenoside Rg1
high pressure liquid chromatography
Renax oral liquids
