摘要
本实验应用放免测定及分子杂交技术观察了香烟烟雾抽提物（ＣＳＥ）对培养的猪肺动脉内皮细胞环氧合酶，血栓素合成酶基因表达和ＰＧＩ＿２，ＴＸＡ＿２生成的影响，进一步从分子水平探讨由内皮细胞产生的前列腺素在ＣＳＥ影响肺血管张力和ＨＰＶ中的作用。结果发现：在常氧或缺氧下，ＣＳＥ可促使内皮细胞环氧合酶—１ｍＲＮＡ水平升高于对照组的１．５倍，而环氧合酶—２ｍＲＮＡ则分别比对照组高２倍和１倍。同时在常氧或缺氧时，ＣＳＥ使内皮细胞条件培养基中６—ｋｅｔｏ—ｐＧＦｌａ．明显高于对照组（Ｐ＜０．０５）。然而，不论常氧或缺氧时，ＣＳＥ不影响血栓素合成酶基因表达和ＴＸＡ２的生成。提示在常氧和缺氧时，ＣＳＥ所致的环氧合酶ｍＲＮＡ水平升高及ＰＧＩ２增加介导了猪吸烟时肺血管张力和ＨＰＶ的降低。
The effect of cigarette smoke extracts(CSE)on gene expression ofcyclooxygenase and thromboxane synthase and production of PGI2 and TXA2 in culturedprocine pulmonary artery endothelial eells were observed,using radioimmuno- assay andNorthem blot technique. Either in normoxia or hypoxia,cyclooxygenase-1 mRNA level wereincreased by 1.5 times with CSE at same extent in comparison with control group,whilecyclooxygenase-2mRNA level were elevated by 2 times and 1 time respectively.The contentof 6- keto-PGF1α in conditioned medium in nomoxia and hypoxia were also increasedsignificantly by CSE,in comparison with control group,However,either in normoxia or inhypoxia CSE did not affect the expression of thromboxane synthase gene and productionof TXA2 These results suggested that the increase of cyclooxygenase mRNA levels and PGI2production in procine pulmonary artery endothelial eells caused by CSE in normoxia andhyp oxia mediated the decrease of pulmonary vascular tone and HPV in pigs duringsmoking.
出处
《中国病理生理杂志》
CSCD
北大核心
1996年第4期415-418,共4页
Chinese Journal of Pathophysiology
基金
国家八五攻关资助课题
