摘要
应用荧光法测定了盐酸小檗碱对白蛋白的荧光猝灭作用；由Ｌｉｎｅｗｅａｖｅｒ－Ｂｕｒｋ双倒数作图法，得出了盐酸小檗碱与白蛋白的解离常数为１．７３×１０￣（－５）ｍｏｌ／Ｌ。
Fluorescence method has been used to observe the fouorescence quenching of human serum albumin (HSA) by its interaction with bcrberine hydrochloride (BH). The interaction dissoriation constants of HSA and BHhave been determined from a double reeiprocal Linewetiver-Burk plot (Kd=1.73×10￣(-5) mol/L).
出处
《中国中药杂志》
CAS
CSCD
北大核心
1996年第3期175-176,共2页
China Journal of Chinese Materia Medica
关键词
盐酸小檗碱
白蛋白
解离常数
荧光法
berberine hydrochloride
human serum albumin
dissociation constant
fluorescence
