摘要
目的探讨褪黑素对实验性糖尿病大鼠肾脏病变的保护作用及其机制。方法将实验动物随机分为正常对照组(C组)、糖尿病组( D组)及糖尿病褪黑素治疗组(M组)。给予褪黑素治疗4周,检测血糖、血肌酐、肾脏肥大指数(肾重/体重) 放免法测定 24 h 尿白蛋白排泄量, 对肾脏标本进行光镜观察, 用图像分析仪测量各组大鼠平均肾小球面积、平均肾小球体积。同时用实时荧光定量 RT- PCR 法对糖尿病大鼠肾皮质转化生长因子β1( TGF-1) mRNA 表达进行半定量分析。结果褪黑素治疗组较糖尿病组 24 h 尿白蛋白排泄量、肾脏肥大指数均明显下降 (P <0.05)。实时荧光定量 RT- PCR 检测糖尿病大鼠肾脏皮质 TGF-β1mRNA 表达明显高于正常对照组,为C组的9.2倍, 给予褪黑素治疗后, 糖尿病大鼠 TGF-β1 mRNA 表达水平下降 3.95 倍, 但仍明显高于正常对照组(P <0.01)。肾小球平均面积、平均肾小球体积 M 组显著低于 D 组(P <0.01)。结论褪黑素可抑制糖尿病大鼠肾脏皮质 TGF- β1 mRNA 表达, 减少糖尿病大鼠的尿白蛋白, 减轻及延缓肾小球硬化, 发挥保护肾脏的作用。
[Objectives] To investigate the mechanism of melatonin in protecting the renal lesion of diabetic rats. [Methods] Experimental diabetic rats were induced by peritoneal injection of streptozotocin. The rats were randomly divided into following groups： normal control rats （group C）, streptozotoein-induced diabetic rats （group D） and diabetie rats treated with melatonin （group M）. After 4 weeks, the serum creatinine, kidney hypertrophy index （kidney weight/body weight ratio）, and 24 h urinary albumin excretion rate were measured, respectively. TGF-β1 mRNA expression in renal cortex of rats using newly real-time quantitative RT-PCR with SYBR Green Ⅰ and the kidney samples were observed with light microscope, mean glomerular transverse sectional area （MGA） and mean glomerular volume （MGV） were also calculated by analysis system of the image. [Results] After 4 weeks, kidney hypertrophy index and 24 h urinary albumin exeretion of melatonin treatment group were signffieantly lower than those in diabetic group D （P 〈0.01）. TGF-β1 mRNA expression levels revealed a 9.2-fold increase in diabetic glomcruli after four weeks compared to normal glomeruli （P 〈0.01）. While ntelatonin treatment decrease 3.95-fold, but still signifocantly higher compared to normal glomeruli （P 〈0.01）. Furthermore, the MGA and MGV in group M were significantly smaller than those of group D （P 〈0.01）. [Conclusion] Melatonin possesses a renal protective effect on diabetie rats, whose mechanism may be associated partly with a down-regulation of the expression of TGF-β1 mRNA.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2006年第6期817-821,共5页
China Journal of Modern Medicine
基金
Youth fund of Shan Dong Province (Project Number :2001CA2EFB1)
关键词
糖尿病肾病
转化生长因子-Β1
实时荧光定量RT-PCR
diabetic nephropathy
transforming growth factor β1
real-time quantitative reverse transcriptasepolymerase chain reaetion （RT-PCR）
