摘要
目的评价血清有机磷酸酯酶（ＰＥ）与肝纤维化的联系。方法用４０％ＣＣｌ４１ｍｌ／ｋｇ体重皮下注射，２次／周，共１４周诱发ＳＤ大鼠肝纤维化，分别于１，２，３，５，７，１０和１４周处死动物，测定血清和肝组织中ＰＥ、磷酸二酯酶Ⅰ（ＰＤＥⅠ）、单胺氧化酶（ＭＡＯ）、结合甘胆酸（ＣＧ）、β２＿微球蛋白（β２＿ＭＧ）、透明质酸（ＨＡ）和Ⅲ型前胶原（ＰＣⅢ）等。结果血清ＰＥ、ＰＤＥⅠ和ＣＧ于染毒后逐渐升高并与肝组织纤维化程度相关，ＡＫＰ和ＧＰＴ也升高，但呈不规则变化。ＭＡＯ，β２－ＭＧ，ＨＡ和ＰＣⅢ无变化。肝组织中羟脯氨酸含量增加，其它指标无变化。结论血清ＰＥ活性与肝纤维化程度密切相关。
AIMS To study the changes of serum phosphonate esterase and other serum enzymes in experimental liver fibrosis in rats. METHODS Liver fibrosis model was inducd in SD rats by injection of 40% CCl 4, 1.0ml/kg body weight, subscutaneously, twice a week for 14 weeks. The animals were sacrificed in 1, 2, 3, 5, 7, 10, 14 weeks respectively. Serum and liver phosphonate esterase (PE), phosphodiesterase Ⅰ (PDE Ⅰ), alkaline phosphatase (ALP), alanine tranasaminase (ALT), hyaluronate(HA) and glycocholic acid (GC) were detected. RESULTS The serum PE, PDE Ⅰ and GC were gradually increased during fibrosis of liver. After 14 weeks of CCl 4 injection, the serum PE, PDE Ⅰ activities in fibrosis group were elevated twofold compared with control group. CONCLUSIONS Serum PE, PDE Ⅰ may be used as new markers for liver fibrosis or cirrhosis.
关键词
肝纤维化
有机磷酸酯酶
血清
liver cirrhosis/enzymology phosphoric monoester hydrolases/blood phosphoric diester hydrolases/blood
