摘要
目的:建立以在线衍生-二极管阵列检测器联用测定复方氨基酸胶囊中7种氨基酸含量的方法。方法:采用高效液相色谱仪带全自动衍生功能的自动进样器,按预先设置的程序进样,并利用二极管阵列-荧光检测器串联分析。结果:7种氨基酸分离较好,且检测浓度分别在一定范围内与各峰面积积分值呈良好线性关系。结论:本方法操作简便、重现性好、灵敏度高、定量准确,可推广应用。
OBJECTIVE： To develop a HPLC assay for the determination of 7 kinds of amino acids in amino acid capsules by using online derivation and diode array detector.METHODS：The high performance chromatograph of liquid with the device of automatic derivation was employed as the automatic sampler.The samples were injected in the predefined procedure, and detected with the diode array detector.RESULTS： Good resolution of 7 kinds of amino acids was obtained, and the respective detectable concentration showed a good linear relationship with its peak area.CONCLUSION： The present method is convenient, well reproduced, highly sensitive, and accurate, therefore it can be widely used.
出处
《中国药房》
CAS
CSCD
北大核心
2006年第6期454-455,共2页
China Pharmacy
