摘要
对3个不同养殖群体裙带菜Undaria pinnatifida(本地裙带菜、日本裙带菜、自育裙带菜)的孢子叶、茎和叶不同部位的过氧化物酶(POD)和酯酶(EST)进行了同工酶电泳分析。结果表明:3个裙带菜群体的POD同工酶其孢子叶活力都相对强于其它部位;日本裙带菜茎和叶的各部位POD酶活力相同,但叶中部的酶活力稍强于茎;自育裙带菜茎底部和茎中部的POD酶活力较强些,而叶底部的酶活力较叶中部和顶部的强;本地裙带菜的茎和叶由底部到顶部POD酶活力逐渐变弱。孢子叶EST酶活力要较茎和叶的酶活力强很多,3种裙带菜孢子叶中EST酶活力相同。此外,还对裙带菜的种质、样品处理方法以及3种裙带菜的亲缘关系进行了讨论。
Peroxidase （POD）and esterase （EST） isoenzymes were analysed in sporophyll, stem and leaf in three populations （ native, Japanese and half - bred ） of sea mustard Undaria pinnatifida by electrophoresis. The results showed that the POD activity was higher in the sporophyll than that in the stems and leaves in which the POD activities were weak from bottom to top in native plant. For the Japanese plant, the POD activities of the stems and leaves were the same in all positions. However, the activity was slightly higher in midportion of a leaf than that in stems. The high enzyme activities were observed in mid - bottom stems in the haftbred plant, and the activity was higher in bottom leaf than that in mid -top leaf. The EST enzyme activity was higher in the sporophyll than that in the stems and leaves. The methods of sample treatment, and relationship among the breeds were discussed.
出处
《大连水产学院学报》
CSCD
北大核心
2006年第1期59-63,共5页
Journal of Dalian Fisheries University
基金
国家质检总局资助项目(LK31-2002)
关键词
POD
EST
同工酶电泳
裙带菜
peroxidase
esterase
isoenzyme electrophoresis
Undaria pinnatifida
