摘要
目的：探讨冻存猪肝细胞移植治疗大白鼠急性肝衰的可行性。方法：将猪肝细胞在液氮中冻存１５０ｄ后移植给切除８０％肝叶的大鼠。结果：冻存肝细胞活率为冻前的７８％，光、电镜显示细胞完整，细胞内糖原和葡萄糖－６－磷酸酶丰富。移植组鼠的存活率为１６／ｌ７，明显高于对照组３／９，Ｐ＝０．００２。但肝衰后２和７ｄ的两组间残肝重量及肝功能生化值并无显著差异。结论：冻存猪肝细胞移植可治疗急性肝衰，其作用机理可能是综合效应的结果。
Objective:The experiment aimed at studying the possibility of transplantation of pig' s cryop-reservated hepatocytes treating acute hepatic failure in Wistar ratx.Methods:Porcine hepatocytes werecryopreserved in liquid nitrogen for 150 days,which were transplanted into the rats in which 80% of liver lobes were resected later.Results:The viability of the cryopreserved cells was 78% of the freshcells.Morphologically intact hepatocytes were observed under light and electron microscopy.Glycogenand glucose-6-phosphatase within the cells were rich.Survival rate of rats in transplant group was 16/17,significantly higher than 3/9 of control group(P=0.0020)But there weren't significant differ-ences in the weights of remaining partx of livers and biochemical values of hepatic function between the 2-day group and 7-day group after liver failure.Conclusions:The transplant of pig's frozen hepatocytescould treat the acute hepatic failure and its mechanixm seemed to be a result of multiple effects.
出处
《北京医科大学学报》
CSCD
1996年第1期4-6,共3页
Journal of Peking University(Health Sciences)
基金
卫生部科研基金
关键词
肝功能衰竭
肝移植
异体移植
组织保存
Headings Liver failure/ther:Liver transplantation
Transplantation,heterologous
Tis-sue preservation
