摘要
目的:探讨人端粒酶逆转录酶(hTERT)及凋亡抑制基因生存素(survivin)在尖锐湿疣(CA)及外阴鳞状细胞癌(VSCC)组织中的表达及其临床意义。方法:SP免疫组化染色检测48例CA、31例VSCC及13例正常皮肤组织中survivin蛋白表达水平;原位杂交技术检测上述组织中人端粒酶逆转录酶(hTERT)mRNA表达水平。结果:CA组hTERT mRNA表达的阳性率与正常对照组比较差异无显著性,与VSCC组比较差异有显著性;CA组、VSCC组及正常对照组中survivin蛋白表达的阳性率差异均有显著性;VSCC组中hTERT mRNA表达与survivin蛋白表达的阳性率呈明显的正相关(P<0.05)。结论:survivin与CA及VSCC的发生发展密切相关;hTERT可作为鉴别良性和恶性肿瘤的辅助指标,但不能反映良性肿瘤的增殖情况;hTERT与survivin在VSCC中的表达具有一定的协同性。
Objective： To measure the expression of survivin and hTERT（ human telomerase reverse transeriptase） and their clinical significance in condyloma acuminatum （CA） and vulvar squamous cell carcinoma （VSCC）. Methotis： The expression of survivin was measured by streptavidin- peroxidase complex technique in 48 cases of CA, 31 cases of VSCC and 13 specimens of normal skin. The expression of hTERTmRNA was measured by in situ hybridization technique. Results： The expression of hTERT in VSCC was significantly higher than that in CA and normal skin, while the expression of hTERT in CA and normal skin was similar. The survivin expression in CA and VSCC was significantly higher than that in normal skin. The hTERT expression was positively correlated with the high survivin expression in VSCC （ P 〈 0.05）. Conclusion： The high expression of survivin may play an important role in the development of CA and VSCC. hTERT may be used as a marker for the differentiation between benign and malignant skin tumors, but it is not related to the proliferation of benign tumors. The expression of survivin and hTERT in VSCC is synergetic.
出处
《中国麻风皮肤病杂志》
2006年第3期179-181,共3页
China Journal of Leprosy and Skin Diseases
关键词
尖锐湿疣
外阴鳞状细胞癌
人端粒酶逆转录酶
生存素
condyloma acuminatum
vulvar squamous cell carcinoma
human telomerase reverse transcriptase
survivin
