摘要
从组织提取RNA的完整性对于分子生物学研究是至关重要的。RNA提取方法有多种。采取异硫氰酸胍法从黄河鲶肌肉、血液、全鱼、肾脏、肝脏、卵巢中提取出完整RNA,结果表明,通过该方法所提取RNA质量高,效果好,可以用于进一步的分子生物学研究。
The integrality of RNA extracted and purified from tissues is essential for molecular biology. Some useful extracting and purifying methods had been discovered. Guanidine Thiocyanate is ideal for extracting and purifying RNA of the Yellow River catfish. Through this method, RNA of high quality had been obtained from muscle, blood, kidney, liver, ovary, which can be used for the further molecular biology research.
出处
《淡水渔业》
CSCD
北大核心
2006年第2期24-26,共3页
Freshwater Fisheries
基金
宁夏回族自治区8613重大农业科技攻关项目(2002-Z01-04)资助
关键词
黄河鲶
RNA提取
异硫氰酸胍法
the Yellow River catfish
RNA extraction
Guanidine Thiocyanate