摘要
目的建立高效液相色谱法测定西替伪麻缓释片中西替利嗪在Beagle犬体内血药浓度并进行生物利用度评估。方法以二氯甲烷提取溶剂,异丙嗪为内标;采用C18柱,以乙腈-0.02 mol/L的醋酸钠水溶液-高氯酸-三乙胺(体积比50∶50∶0.5∶0.1),用5 mol/L的氢氧化钠调节pH 5.2为流动相,检测波长230 nm。结果与结论本文方法提取率高,回收率好,自制片的相对生物利用度93.0%。
Objective To establish an HPLC assay for the determination of blood plasma concentration of cetirizine in cetirizinepseudoephedfine sustained-release tablets in beagle dogs. Method Cetirizine was extracted by methylene chloride. Promethazine was used as an internal standard and the separation was performed on a reversed phase Diamonsil C18 （5μm,250 mm × 4.6 mm） employing a UV-detection set at 230 nm at ambient temperature. The mobile phase consists of acetonitrile-0.02 mol/L acetate water solution- perchloric acid-triethylamine （50： 50： 0.5：0.1 ） adjusted to pH 5.2 with 5 mol/L NaOH. Result and Conclusion The assay is linear from 50 to 3200 ng/ml with a detection limit of 15 ng/ml. The relative bioavailability of cetirizine in the tablets was 93.0%.
出处
《广东药学院学报》
CAS
2006年第1期10-12,共3页
Academic Journal of Guangdong College of Pharmacy
基金
广东省科技厅资助项目(2005B30101002)
关键词
西替伪麻缓释片
高效液相色谱法
药代动力学
生物利用度
cetirizine-pseudoephedrine sustained-release tablets
HPLC
pharmacokineties
bioavailability
