摘要
目的:探讨革兰阴性杆菌感染患者和正常健康人群血浆内毒素水平及临床意义。方法:分别用定量动态比浊法和鲎试剂定性凝胶法检测革兰阴性菌感染患者和正常健康对照血浆内毒素含量。结果:正常对照组血浆内毒素定量3.30±1.73pg/ml,定性全部为阴性;革兰阴性菌感染组内毒素945±2244.43pg/ml,定性阳性率为68.19%。经SPSS统计软件T-检验分析,对照组与革兰阴性菌感染组内毒素平均值差异显著(t=2.608,P=0.025),相关性分析表明定量动态比浊法和鲎试剂定性凝胶法在0.01水平相关。结论:检测血浆内毒素含量有助于早期判断感染的细菌种类及是否存在内毒素血症,有利于临床合理应用抗菌药物和及时对症处理。
Objective：To study the clinical significance of plasma endotoxin detecting of patient with gram negative bacillus infection.Methods： Plasma endotoxin of patient and healthy people were detected by turbidimetric assay and gel - clot assay with limulus reagents. Results： Normal plasma entoxin of healthy people was 3.30 ± 1.73pg,/ml and gel- clot assay were all negative. In gram negative bacillus infection group, the result was 945 ± 2244.43 pg/ml and positive rate of gel- clot assay was 68.19% .T- test analysis indicates the mean plasma entoxin of two groups have notable difference（t = 2.608, P = 0.025 ）. Relativity analysis indicates them was correlation between turbidimetric assay and gel- clot assay on 0.01 level. Conchusion ： Plasma endotoxin detecting is helping to estimate what kind of bacteria infected and endotoxemia. It is also significant to select antibacterial reagents nationally and handle syndrome on time.
出处
《航空航天医药》
2006年第1期1-2,共2页
Aerospace Medicine
