摘要
为研究狂犬病毒基因的结构,将狂犬病毒HEP-Flury株全基因组cDNA与N、P、L、G 4个基因的辅助质粒共转染BHK-21细胞,于体积分数为5%CO2培养箱中培养6d,以狂犬病毒N蛋白荧光抗体染色。结果显示,细胞中含有大量的狂犬病毒粒子,表明已建立了狂犬病毒的反向遗传操作系统。
To study the genes of Rabies virus, the full-length genome cDNA of the HEP-Flury strain and four helper plasmids with N, P, L and G gene respectively were co-transfected to BHK-21 cell. After incubation in 5% CO2, the cells were stained with fluorescence antibody of anti-N protein MAb. The results showed that the rabies virus was efficiently recovered from the clone eDNA and Rabies Virus reverse genetic system was developed.
出处
《动物医学进展》
CSCD
2006年第2期99-100,共2页
Progress In Veterinary Medicine
基金
广东省自然科学基金(5200638)
