摘要
目的建立替考拉宁组分和有关物质测定的HPLC法。方法使用YWGC18柱(200m m×4.6mm,10μm),流动相为0.02molL/磷酸二氢钠(pH 6.0)∶乙腈(73∶27),流速1.0mlm/in,检测波长230nm。结果替考拉宁在浓度70～430μg/m l内与峰面积呈良好的线性关系,r=0.9999,平均回收率98.93%,RSD为0.86%(n=9)。结论该方法快速、简便、准确,可用于替考拉宁组分及有关物质测定。
Objective To establish an HPLC method for content determination of teicoplanin and its related substance. Methods A C18 column（YWG, 200×4.6mm, 10μm）was used; mobile phase： 0.02mol/L phosphate buffer （pH6.0）： acetonitrile （73：27）; flow rate： 1.0ml/min; wavelength： 230nm. Results The linear range for teicoplanin was 70～430μg/ml （r=0. 9999）. The average recovery of 98.93% was obtained with a RSD of 0. 86% （ n = 9）. Conclusion The method was rapid, simple and accurate for content determination of teicoplanin component and its related substances.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2006年第3期184-186,192,共4页
Chinese Journal of Antibiotics
