摘要
采用缺陷型逆转录病毒转导人ＴＮＦ－α基因至口腔癌ＤＮＬ，并应用ＰＣＢ技术、ＴＮＦ－α活性检测技术、Ｇ４１８筛选试验对ＤＮＬ中外源基因及其上清液中的ＴＮＦ－α活性进行了检测。结果：１．转导基因的ＤＮＬ中有外源基因存在；２．转导基因的ＤＮＬ上清液中有高活性的ＴＮＦ－α；３．转导基因ＤＮＬ能在Ｇ４１８培养基中生长。表明缺陷型逆转录病毒载体能够将ＴＮＦ－α基因导入口腔鳞癌ＤＮＬ中表达，转导基因ＤＮＬ能够分泌高活性的ＴＮＦ－α。该结果为在口腔癌领域开展细胞因子基因治疗奠定了基础。
The gene coding for TNF-α was introduced into DNL of oral squamous cell carcinoma with retroviral-mediated gene transduction,TNF-α gene and TNF-α production was detected.The results showed:1.With PCR technique,exogenous gene found in transduced DNL;2.TNF-α production was found in supernatant of transduced DNL;3.Transduced DNL could grow in G418 medium. The test demonstrated that the gene coding for TNF can be introduced into DNL of oral squamous cell carcinoma with retfoviral-mediated gene transduction.This study suggest that TNF-α gene transduction may be important strategic approach to therapy of oral cancer.
出处
《上海第二医科大学学报》
CSCD
1996年第2期77-79,共3页
Acta Universitatis Medicinalis Secondae Shanghai
基金
国家八六三课题基金
国家自然科学基金
关键词
逆转录病毒载体
基因转移
TNF-Α基因
鳞癌
retroviral vector
gene transduction
TNF-α gene
oral squamous cell carcinoma
lymphocyte
