摘要
目的:研究乌司他丁对心肺转流(CPB)心脏手术中缺血一再灌注损伤的器官保护作用。方法:择期CPB心内直视手术20例随机均分为乌司他丁组(U组)和对照组(C组)。U组分别于阻断主动前和开放主动脉后给予乌司他丁各5 000μ/kg,C组同时段给予生理盐水,分别于麻醉诱导,阻断主动脉30 min,开放主动脉30 min,开放主动脉1 h,开放主动脉2 h采集静脉血,用ELISA法测定样本中肿瘤坏死因子-α(TNF-α),白细胞介素-6(IL-6),白细胞介素-8(IL-8)的水平。结果:两组中各项指标在麻醉诱导期无显著差异(P>0．05);两组中各项指标在阻断主动脉30 min,开放主动脉30 min、1 h、2 h与麻醉诱导期比较有明显增加(P<0．05和P<0．01);U组中血浆TNF-α、IL-6、IL-8的浓度在阻断主动脉30min, 开放主动脉30 min,1 h、2 h与C组比较有显著差异(P<0．05)。结论:乌司他丁能抑制机体炎症反应,减轻缺血-再灌注损伤,从而达到保护重要脏器的作用,但不能完全阻断炎症反应所致的中性拉细胞增加。
Objective： To explore the protective effect of ulinastatin on the organs of ischemia-reperfusion injury during cardiac surgery with CPB. Methods： 20 patients scheduled for selective open-heart surgery with CPB were divided randomly into Group U （treated with ulinastatin） and Group C （control group）. Group U was given 5000u/kg respectively before aortic clam ping and aortic unclamping while Group C was given NS. Venous blood was collected to determine the levels of tumor necrosis factor-α （TNF-α）, interleukin-6 （IL-6） and interleukin-8 （IL-8） with ELISA at the following time points： anesthesia induetion, 30rain after aortic clamping, 30min after aortic unclamping, lh after aortic unclamping, 2h after aortic unclamping. Resuits： There is little difference between all the items of both groups at the time of anesthesia induction（P〈0. 05）; there is a great increase at the time points of 30min after aortic clamping, 30min, 1h, 2h after aortic unclamping （P〈0. 05 and P〈0. 01 ）; The concentration of INF-α, IL-6, IL-8 of group U differs greatly at the time points of 30 min after aortic clamping, 30 min, 1h, 2h after aortic unclamping, compared with that of Group C（P〈0. 05）. Conelusion：Ulinastatin has the protective effect on the important organs by controlling the inflammation and alleviating ischemia-reperfusion injury, but it can not stop the increase of neutrophil caused by inflammation.
出处
《中国临床医学》
北大核心
2006年第1期149-150,共2页
Chinese Journal of Clinical Medicine
关键词
乌司他丁
心肺转流
脏器损伤
细胞因子
Ulinastatin
Cardiopulmonary bypass
Organ injury
Cytokinin
