摘要
目的:探讨颗粒酶B(granzyme B,GrB)在原发性肝细胞癌(HCC)、癌旁、肝硬化及正常肝组织中的表达,及GrB+淋巴细胞与HCC、肝硬化的关系。方法:HCC60例,单纯性肝硬化62例,正常肝组织23例,以免疫组化二步法进行GrB染色,对阳性细胞数进行定量分析。结果:①各组GrB+细胞平均数从高到低为:癌旁,HCC,正常肝,肝硬化组织;②HCC中 GrB+细胞与组织学分级无明显关系:(P>0．05);③HCC中15月内有转移组的GrB+细胞数明显少于无转移组:(P<0． 01);④HCC中GrB+细胞随着临床分期的发展有下降的趋势:(P<0．05);⑤以术前甲胎蛋白(AFP)阴、阳性分组(20μg／L 为标准),AFP阴性组和AFP阳性组两组数据无明显差异:(P>0．05);⑥肝硬化和癌旁组中乙型肝炎表面抗原(HBsAg) 阳性组GrB+细胞均多于阴性组:(P<0．05);HOC组GrB+细胞与HBsAg无密切相关:(P>0．05);⑦癌旁GrB+细胞与是否伴肝硬化无明显关系:(P>0．05)。结论:临床上,随着HCC患者的病情恶化,GrB+细胞逐渐减少。GrB+细胞数量与HBsAg密切相关,与组织学分级、AFP和是否伴有肝硬化无关。GrB+细胞也可成为反映机体抗肿瘤特异性细胞免疫状态及判断患者预后的重要指标之一。
Objective： To investigate the expression of granzyme B（GrB） in hepatocellular carcinoma（HCC）, paraneoplastic, cirrhosis and normal liver tissues and the relationship between GrB^＋ Iymphocytes and HCC patients situation in clinic. Methods： Surgical specimens from 60 cases of HCC, 62 cases of cirrhosis and 23 cases of normal liver tissues were investigated by immunohistochemicaI staining of GrB with polymer detection system. Results： ①The order of the number of GrB^＋ cells from the highest to the lowest was： paraneopIastic, HCC, normal liver and cirrhosis tissues;② There was no relationship between the number of GrB^＋ cells in HCC and its histological grade; ③ The number of GrB^＋ cells in the cases with metastasis in 15 months was significantly lower than that without metastasis;④ The number of GrB^＋ cells in HCC deaeased with the clinical TNM stage;⑤There was no statistical difference between the data of AFP group progress of negativebefore operation and that of positive;⑥The number of GrB^＋ cells of HBsAg positive group was higher than that of the negative one in cirrhosis and paraneoplastic tissues; There was no relationship between the number of GrB^＋ cells and HBsAg in HCC;⑦There was no statistical difference between the data of the group with cirrhosis and that without cirrhosis in paraneoplastic tissues. Conclusion：The number of GrB^＋ cells decrease with the progress of the HCC patients in clinic. The number of GrB^＋ cells is closely related to HBsAg, meanwhile it possess no relevance to the pathological grades, AFP level and cirrhosis. The number of GrB^＋ cells might be an important marker to estimate the local immune status and a useful factor to predict the prognosis of HCC patients.
出处
《中国临床医学》
北大核心
2006年第1期72-74,共3页
Chinese Journal of Clinical Medicine
基金
广西科学基金资助(合同编号:桂科0342021)
关键词
颗粒酶B
原发性肝细胞癌
肝硬化
NK细胞
T细胞
Granzyme B
Hepatocellular carcinoma
Cirrhosis
Natural killer cell
Cytotoxic T lymphocyte
