摘要
将4-羟基-2,2,6,6-四甲基哌啶氮氧自由基用于标记9羧基吖啶,得到自旋标记荧光探针4-(9-吖啶酯)-2,2,6,6-四甲基哌啶氮氧自由基.以谷胱甘肽作为蛋白质肽模型,研究了活性氧过氧亚硝酸诱导其损伤产生的硫中心自由基的荧光表征.自旋标记荧光探针为弱荧光物质,当与硫中心自由基作用后,导致其荧光增强,从而可对硫中心自由基进行表征.
A spin-labeling fluorophore（Ac-Tempo ）, formed by combining with an acridine with a paramagnetic nitroxide （4-hydroxyl-2,2,6,6-tetramethylpiperidinyl-1 -oxy）, was explored for peptide-based thiyl radicals generated through the reaction between gluththione and peroxynitrite. Ac-Tempo＇ , a weak fluorescent compound, showed a dramatic increase in fluorescence intensity after interaction with thiyl radicals, and could be employed to probe peptide-based thiyl radicals.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第3期424-427,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20375032)资助.
