摘要
目的了解膀胱灌注辣椒辣素后局部的组织学反应。方法选用66只雌性SD大鼠，体重201—225g。随机分成对照组（n=18），100μmol／L辣椒辣素膀胱灌注组（n=24）和2mmol／L辣椒辣素膀胱灌注组（n=24）。辣椒辣素经尿道插管注入膀胱。大鼠分别于1h（n=6），1周（n=6），4周（n=6）和8周（n=6）处死，取出膀胱，测湿重并行光镜检查。结果3组问及各组不同时间膀胱湿重差异均无统计学意义（P〉0．05）。100μmol／L组镜下仅显示固有层表面轻度的血管扩张；2mmol／L组可见膀胱黏膜糜烂，水肿，固有层毛细血管扩张及肌层表面的局灶性损伤。黏膜层的改变于1周后开始修复。8周后黏膜层恢复正常。结论对大鼠行100μmol／L辣椒辣素膀胱灌注是一种安全的治疗方法。过高剂量的辣椒辣素可引起可逆性的黏膜损伤和炎性改变。
Objective To investigate the histopathologic features of urinary bladder with capsaiein intravesical instillation in rats. Methods A total of 66 female Sprague-Dawley rats （body weight,201 - 225 g） were randomly divided into 3 groups, ie, control（ n = 18 ） , capsaicin 100μmol/L（ n=24） and capsaicin 2 mmol/L（ n = 24）. Capsaicin intravesical instillation was done via a urethral catheter after dilution in saline and alcohol. The animals were killed by CO2 after 1 h （n = 6 ） , 1 week （n =6 ） ,4 weeks （n=6 ）and 8 weeks（n = 6） in each group. The bladder wet weight was measured, and light microscopy examination was performed in each sample. Results After capsaicin instillation,there were no significant differences of bladder wet weight at various time points among 3 groups （ P 〉 0.05）. Morphology showed slight dilation of the vessels in the superficial part of lamina propria in the 100μmol/L capsaicin group. In the group treated with 2 mmol/L capsaicin,erosion of the mucosa,edema and capillary distention in the lamina propria were seen as well as focal damage to the superficial muscle near the lamina propria. This led to hematuria in the early stage. The mucosal changes started to heal after 1 week and returned to normal after 8 weeks. Conclusions In rats,intravesical capsaicin instillation at a dose of 100μmol/L appears to be a safe method of treatment. High doses of capsaicin may result in some reversible damages of the mucosa and inflammatory changes of the bladder.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2006年第3期181-183,共3页
Chinese Journal of Urology
