摘要
目的检测不同雌激素水平对大鼠甲状腺组织碘摄取及NIS基因表达的影响。方法雌性Wistar大鼠48只,随机分为去卵巢组、假手术对照组、小剂量雌激素治疗组、大剂量雌激素治疗组4组,运用131I及99mTcO4核素扫描观察各组甲状腺碘摄取功能,用RT-PCR法检测甲状腺组织NIS基因的表达。测定各组大鼠TSH、FT3、FT4、雌激素水平,并进行甲状腺组织病理检查。结果大剂量雌激素对甲状腺NIS的表达水平有明显的抑制,而去卵巢组NIS的表达亦低下。在整体水平上,虽然雌激素抑制了NIS表达,导致甲状腺滤泡细胞碘转运功能下调,但其仍增强了甲状腺对碘的氧化聚集功能,增强碘摄取,并促进了甲状腺滤泡细胞的增大和生长。结论雌激素抑制NIS的表达,但在整体水平上增强甲状腺碘的摄取功能,并促进甲状腺细胞生长,在甲状腺肿疾病的发生中有重要作用。
Objective To identify the impact of estradiol on the goiter and the function of the thyroid gland with different dosages of estradiol. Methods 48 Wistar rats （female, normal state） were divided into 4 groups： Ⅰ , ovx, Ⅱ , sham; m ,ovx ＋E2- low and Ⅳ ovx ＋ E2-large. In each group, we applied nuclide scanning of ^131I and ^99mTcO4 to detect the thyroid iodine uptake and adopted the technique of RT-PCR to test the expression of NIS. Quantity of TSH,FT3 ,FT4 and the estradiol were examined. Results The expression of NIS was inhibited in group Ⅰ and group Ⅳ. Conclutions Estradiol can enhance the capability of the thyroid glands uptaking iodine and inhibit the expression of NIS and so estradiol is a very important component in the pathogenesis of goiter.
出处
《基础医学与临床》
CSCD
北大核心
2006年第2期177-181,共5页
Basic and Clinical Medicine
