摘要
在室内检测了17种紫外保护剂和4种组合对3种昆虫病原真菌即球孢白僵菌Beauveria bassiana、金龟子绿僵菌Metarhizium anisopliae、蜡蚧轮枝孢Verticillium lacani分生孢子的保护作用.结果表明,苯并三唑类2#的紫外保护作用最好,用紫外杀菌灯照射30 min,苯并三唑类2#对3种菌株孢子的保护效率均达90%以上;苯并三唑类3#、苯甲酮类2#、荧光黄钠、刚果红的保护效率可达60%.组合使用紫外保护剂并不能增强保护效果.选用苯并三唑类2#时,适宜的溶剂是花生油加正己烷(1:1,v/v),使用浓度应大于0.75%.用254、312、365 nm波长紫外灯照射40 min内,1%苯并三唑类2#对3种菌株孢子的保护效率可保持90%,照射180 min后,保护效率在56%～77%.
The UV-screen effect of 17 UV-protectants and 4 combinations for conidia of Beauveria bassiana, Metarhizium anisopliae and Verticillium lacanii was inspected in laboratory. UVP-2 （benzotriazole 2^#） was confirmed to be the most effective protectant. Protective efficiency of UVP-2 to spores of entomopathogenic fungi was over 90%, and other 4 UVPs （benzotriazole 3^#, oxybenzone 2^#, sodium uranine and Congo red） were about 60%, after 30 min exposure of spores to UV. Mixture of UVPs did not show higher effectiveness compared to single UVP. The mixture for UVP-2. The effective concentration of UVP-2 should be of peanut oil and n-hexane was suitable solute higher than 0.75% for the protection of fungal conidia. After 40 min and 180 min exposure of fungal conidia to UV light （at λ254nm, λ312nm or λ365nm）, the protective efficiency of UVP-2 could be increased over 90% and 56% -77%, respectively.
出处
《植物保护学报》
CAS
CSCD
北大核心
2005年第4期402-406,共5页
Journal of Plant Protection
基金
中国农业科学院中韩农业合作研究项目(1999)资助
