摘要
目的:探讨一种快速准确普查及诊断鼻咽癌(NPC)的方法。方法:应用聚合酶链反应(PCR)技术对临床怀疑NPC的144例患者的鼻咽组织和鼻咽脱落细胞中EB病毒(EBV)DNA进行检测,并与病理诊断对照。结果:PCR技术检测鼻咽组织中EBV DNA诊断NPC的敏感性和特异性分别为92.16%和80.95%;检测鼻咽脱落细胞中EBV DNA诊断NPC的敏感性和特异性分别为86.27%和71.43%,其差异均无统计学意义(均P>0.05)。结论:检测鼻咽脱落细胞中EBV快速、简单,患者痛苦小,敏感性和特异性均较高,可用于NPC的诊断;对NPC的普查及流行病学调查尤其方便、实用,值得推广。对凡是EBV检测阳性的患者均应提高警惕,追踪观察。
Objective：To develop a method for rapid screening and primary diagnosis of the nasopharyngeal carcinoma （NPC）. Method.. We detected the Epstein Barr virus （EBV） DNA from nasopharynx tissue and desquamative cells in 144 suspicious NPC patients using polymerase chain reaction （PCR）. The results were compared with the pathologic diagnosis. Result： The sensitivities were 92. 16% and 86. 27% respectively, the specificities were 80.95 % and 71.43% respectively. There was no significant difference between the results determined by the EBV DNA from nasopharynx tissue and desquamative cells using PCR. Conclusion： The detection of EBV DNA from nasopharynx tissue and desquemative cells of the NPC by PCR is a simple, safe, rapid, specific and sensitive technique. It is useful in screening, primary diagnosis, evaluation of the curative effect of the NPC.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2006年第4期153-154,共2页
Journal of Clinical Otorhinolaryngology
关键词
聚合酶链反应
爱泼斯坦-巴尔病毒
鼻咽肿瘤
Polymerase chain reaction, Epstein-Barr virus, Nasopharyngeal neoplasms
