摘要
目的研究功能性矫治器引导大鼠下颌前伸后咀嚼肌中基质金属蛋白酶(MMPs)mRNA的表达,探讨功能性颌骨矫形过程中咀嚼肌适应性变化的生物学机制,为矫形治疗提供新的理论依据。方法分别采用RT-PCR法、实时荧光定量PCR方法观察戴矫治器(实验组)和不戴矫治器(对照组)3、7、14、21 d大鼠二腹肌前腹中MMP-2、MMP-9mRNA表达和表达差异。结果①实验组和对照组大鼠二腹肌前腹中MMP-2、MMP-9 mRNA均有表达。②MMP-2mRNA、MMP-9 mRNA表达差异:实验组3、21 d后表达增加,其中21 d较14、7 d表达明显增加;而对照组21 d较143、d表达明显增加。结论①MMP-2、MMP-9在mRNA水平参与了咀嚼肌正常的生长发育过程;②功能性矫治器引导大鼠下颌前伸后咀嚼肌中MMPs在mRNA水平参与了咀嚼肌的适应性变化。
Objective To investigate molecular changes in growing rats masticatory muscle after functional mandibular protrusion. Methods SD rats were randomly divided into eight groups as control 3 d, 7 d,14 d, 21 d and experimental 3 d, 7 d, 14 d, 21 d. The RT-PCR and qPCR methods were used to find expression and any differences of mRNA of MMPs in growing rats masticatory muscle after functional mandibular protrusion. Results ① Expression of MMP-2, MMP-9 mRNA expressed in all growing rats masticatory muscle. ② Expression differences of MMP-2 and MMP-9 mRNA in 3 d, 21 d were found between control and experimental groups after functional mandibular protrusion. Conclusion ① MMP-2 and MMP-9 mRNA participate in masticatory muscle normal development.② MMPs mRNA involve changes in growing rats masticatory muscle after functional mandibular protrusion.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2006年第1期86-89,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
院青年基金资助项目
