摘要
目的:研究L02细胞移植到具有正常免疫活性的大鼠肝内的存活情况.方法:SD大鼠出生前宫内腹腔注射正常人L02肝细胞,诱导胎鼠对人L02肝细胞产生免疫耐受,出生2wk时经脾移植DiI染色后的人L02肝细胞,建立人鼠嵌合肝动物模型.采用免疫荧光、SP免疫组化、DiI荧光示踪等方法,分别检测人白蛋白、特异性人增殖细胞核抗原PCNA(proliferatingcellnuclearantigen)以及在荧光显微镜下观察人L02肝细胞在鼠肝内的分布.结果:于移植后1,2,4,6,8,10wk在荧光显微镜下观察到人L02肝细胞在鼠肝内的动态分布;移植后2,4,6,8wk大鼠均检测出人白蛋白,4wk时分泌最多;移植后2,4,6wk检测出特异性人增殖细胞核抗原PCNA,以4wk发现PCNA阳性细胞最多.结论:移植的人L02肝细胞在具有正常免疫活性的免疫耐受鼠体内能够存活、增殖,并产生人白蛋白.
AIM： To investigate whether human L02 hepatocytes can survive after implantation to the normal, immunocompetent rats.
METHODS： Human L02 hepatocytes were injected into the peritoneal cavities of fetal Sprague Dawley rats to induce immune tolerance to human L02 hepatocytes. After DiI staining, the hepatocytes were implanted to the 2-week rats via the spleen, Imrnuno-fluorescent staining, SP immunohistochemistry, and DiI staining were used to detect human albumin and specific proliferating cell nuclear antigen （PCNA） in the rat liver. The distribution of human L02 hepatocytes was observed under fluorescent microscope.
RESULTS： Dynamic distribution of human L02 hepatocytes in the rat livers was observed from 1^st to 10^th week after implantation. Human albumin was detected at 2, 4, 6, and 8 wk, and the most content appeared at 4 wk. Specific human PCNA was detected in the rat livers from the 2nd to 6th week after implantation. There were the most PCNA positive cells at the 4th week.
CONCLUSION： Human L02 hepatocytes can survive for 10 wk and proliferate after implantation to the normal, immunocompetent rats.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第1期19-24,共6页
World Chinese Journal of Digestology
基金
国家自然科基金资助项目
No.30271173~~
