摘要
目的探讨脑出血后血肿周围脑组织炎性损伤的病理发展过程。方法健康Wistar大鼠48只随机分成脑出血组、对照组。分别于制模后3h、24h、72h、7d取血肿周围脑组织检测细胞间黏附分子(ICAM-1)的表达,并对出血侧脑组织含水量进行测定。结果脑出血组各时相点出血周围组织均有ICAM-1阳性反应的血管和神经细胞,与对照组比差异有极显著性(P<0·01);与对照组比较,脑出血3h组血肿周围组织含水量即开始升高,24h明显增加(P<0·01),72h仍高于对照组,7d时接近对照组。结论ICAM-1在血肿周围的高表达可能是脑出血后血肿周围组织受损的主要原因之一,ICAM-1促进白细胞浸润,释放大量氧自由基,继而导致脂质过氧化加重脑组织损伤,并参与脑水肿的形成发展。
Objective In order to explore pathogenesis about inflammation injury of the hematoma peripheral zone after cerebral hemorrhage. Methods 48 adult male wistar rats are randomly divided into intracerebral hemorrhage （ICH） groupand control group. After 3 h, 24 h,72 h and 7 d of ICH, intercellular adhesion molecule-1 （ ICAM-1 ） was detected by immunohistochemical SABC staining in rats brain slice. Positive cells expression of the ICAM-1 were measured by color image analysis system. Brain edema were assayed by dry-wet weight method, Results The expression of ICAM-1 increased at3 h （P 〈0.01） ,peaked at72 h（P 〈0.01） ,and continued to7 d, （P 〈0. 05 ） , the brain water content began to increase at 3 h, increased markedly at 24 h, still be higher than that at 72 h, but without significant change at 7 d （ P 〉 O. 05 ） compared to contral group. Conclusions The overexpression of ICAM-1 may play important role in the perihematoma edema formation and ischemic injury.
出处
《临床神经病学杂志》
CAS
北大核心
2006年第1期67-69,共3页
Journal of Clinical Neurology
关键词
脑出血
血肿周围组织
细胞间黏附分子-1
含水量
intracerebral hemorrhage
immunohistochemistry
intercellular adhesion molecule-1
edema
