摘要
采用Ｗｈｉｔｅｓｉｄｅ方法从１９例ＲＣＣ中成功地分离到１７例ＴＩＬ，经１９天培养，ＴＩＬ数量平均达１．０４×１０^（１１）个细胞。杀伤自体肿瘤活性为５７．８７±５．８８％，对Ｒａｊｉ细胞的杀伤活性为３７．３９±１０．８８％，两组间比较差异有非常显著性（Ｐ＜０．０１）。明显高于外周血淋巴细胞（ＰＢＬ）的杀伤肿瘤活性（２２．３９±８．８４％）。ＴＩＬ中ＣＤ_３和ＣＤ８细胞分别为６９．９３±６．８０％及４０．２１±６．８０％，均明显高于ＰＢＬ中的ＣＤ３和ＣＤ_８细胞（Ｐ＜０．０１）。ＴＩＬ对Ｋ５６２细胞的杀伤活性为２６．８９±１２．７９％，提示活化的ＴＩＬ有显著的杀伤肿瘤活性，且对自体肿瘤细胞的杀伤活性更为显著，其杀伤活性主要由Ｔ淋巴细胞介导。
Tumor infiltrating lymphoctytes(TIL)suspensions were successfully isolated with the Whiteside method from 19 renal cell carcinoma(RCC).After cultured for an average of 19 days(13￣28 days),the mean TIL recovery was 1.04 ×10￣(11) pertumor.The cytotoxic activity of TII against autologous tumor cells was signifi cantly higher than that against Raji cells(P< 0.01).The latter was however significantly higher than that of peripheral blood lymphocytes(PBL)(P< 0.01). Phenotype studies showed that the mean percentage of CD_3 cells was 69. 93 % and CD8 cells 40.21% both of which were significantly higher than those in PBL(P<0.01). The results denoted that activated TIL has relatively specific antitumor activity against autologous RCC,mediated mainly by T lymphocytes, and the antitumor activity is stronger than that of PBL.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1996年第2期73-76,共4页
Chinese Journal of Urology
关键词
肾肿瘤
癌
T淋巴细胞
免疫疗法
Kidney neoplasms,Carcinoma,T lympocytes,Immunotherapy
