摘要
研究了从肌红蛋白含量较高的金枪鱼加工废弃物碎鱼肉中提取氧合肌红蛋白,得到了提取的最佳实验条件:样品体积2.5倍的0.01 mol/L pH 8.6 Tris-HCl缓冲液、提取1.5 h、硫酸铵饱和度90%盐析等,并经透析、DEAE-Sepharose FF离子交换柱进行了初步的层析纯化,得到蛋白质量分数达87.11%的肌红蛋白。
The Myoglobin was extracted from drossy meat of Tuna. The optimal conditions were determined through experiments and shown as followings： ratio of Tris-HCl buffer（pH 8. 6） to sample of 2.5 v/m, extraction for 1.5 hours, utilization of 90% ammonium sulfate for salting out,etc. Primary purification was carried out with good results.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2006年第1期96-98,126,共4页
Journal of Food Science and Biotechnology
关键词
肌红蛋白
提取
纯化
Myoglobin
extraction
purification
