摘要
目的研究经颅磁刺激(TMS)对脑梗死大鼠梗死侧皮质c-Fos、脑源性神经营养因子(BD-NF)表达的影响。方法采用80只SD大鼠制作一侧永久性大脑中动脉闭塞的脑梗死模型,随机分为模型组和TMS组。模型组自由饲养;TMS组于动物清醒后当天即给予每天2次、每次30个脉冲的TMS治疗(频率为0.5Hz,场强为1.33T)。各组在大鼠脑梗死后1,7,14,21和28d(每时间点8只)检测梗死侧大脑皮质c-Fos、BDNF的表达。结果模型组和TMS组梗死灶周围皮质均可见c-Fos和BDNF的阳性表达,与模型组相同时间点比较,TMS组7,14,21和28d时c-Fos表达较高,差异有统计学意义(P<0.05),7,14和21d时BD-NF表达较高,差异有统计学意义(P<0.01)。结论TMS能促进大鼠脑梗死后梗死侧皮质c-Fos和BDNF的表达,从而发挥其脑保护及康复治疗效应。
Objective To study the effects of transcranial magnetic stimulation （TMS） on the expression of c- Fos and brain-derived neurotrophic factor （BDNF） of the cerebral cortex in rats with cerebral infarction. Methods Cerebral infarction models were established by using of left middle cerebral artery occlusion （MCAO） and randomly divided into a model group （n =40） and a TMS group （n =40） , in additional, TMS treatment （2 times per day, 30 pulses per time） with frequency of 0.5 Hz and magnetic field intensity of 1.33 Tesla was carried out in TM5 group after infarction. The expression of c-Fos and BDNF was measured at 1 d, 7 d, 14 d, 21 d and 28 d after infarction, respectively. Results The positive expression c-Fos and BDNF was detected in the cortex around the infarction areas, while the expression of c-Fos and BDNF was increased significantly in TMS group in comparison to thosc in model group at 7 d, 14 d, 21 d, 28 d （P〈0.05） and7 d, 14 d, 21 d after infarction （P〈0.01）, respectively. Conclusion TMS might have protective effects and rehabilitative effects on cerebral infarction by promoting the expression of c-Fos and BDNF of the cerebral cortex in rats with cerebral infarction.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2006年第2期86-89,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
经颅磁刺激
脑梗死
C-FOS
脑源性神经营养因子
Transeranial magnetic stimulation
Cerebral infaretion
c-Fos
Brain-derived neurotrophie factor
