摘要
背景与目的bcl-2和bax是调控细胞凋亡的主要因素,两者均为野生型p53的下游基因。本研究旨在分析肿瘤相关基因蛋白bcl-2、bax、P53、C-erbB-2和nm23-H1在肺神经内分泌癌中的表达与肿瘤生物学特点及患者生存时间的关系。方法采用HE染色及免疫组化SP法,对59例肺神经内分泌癌患者的预后和免疫表型进行分析,采用χ2检验、Kaplan-Meier生存分析和Cox风险模型分析。结果bcl-2、bax、P53、C-erbB-2、nm23-H1蛋白的阳性率分别为46%、56%、27%、14%、90%。bcl-2表达与肺神经内分泌癌类型、TNM分期及淋巴结有无转移有关(P值分别为0.037、0.011及0.020),但与肿瘤大小无明显相关性;bax表达则与肿瘤大小、种类、分期及淋巴结转移情况均无密切关系。单因素生存分析显示bcl-2、P53均与生存时间呈负相关(P值分别为0.0338和0.0375),nm23-H1与生存时间呈正相关(P值为0.0021)。Cox多因素生存分析发现,只有bcl-2与生存时间密切相关(P值为0.011)。结论bcl-2是肺神经内分泌癌独立的预后因子。
Background and objective bcl 2 and bax are main regulators of cell apoptosis, both are downstream genes of wild-type p53 gene. This study is to investigate the expression of bcl-2, bax, P53, C-erbB-2 and nm23-H1 in neuroendocrine carcinomas of the lung （NEC） and their relationship to tumor biological characteristics and survival time of patients. Methods Hematoxylin-eosin staining and SP immunohistochemical staining were performed on paraffin embedded tissues of 59 patients with NEC, The relationship between the expression of p53, bcl-2, bax and the clinicopathological factors was analyzed by Chi-square test, multivariate analysis and Kaplan-Meier method. Results The following positive expression rates were observed in cancer tissue： bax 56%, bcl-2 46%, P53 27%, C-erbB-2 14%, nm23-H1 90%. Of them bcl-2 related to pathological type （ P = 0. 037）, TNM stage （ P = 0.011 ）, and lymph node metastasis （ P = 0. 020）, bax didn＇t relate to these biological characteristics. Both bcl-2 and P53 showed negative correlation to prognosis in Kaplan-Meier survival analysis （P=0. 0338 and 0. 0375 respectively）, the expression of which linked to poor prognosis and short survival time, while nm23-H1 showed positive correlation to prognosis （P=0. 0021 ）, but Cox survival analysis demonstrated that only bcl-2 was associated with survival time of patients （P： 0.011 ）. Conclusion bcl-2 is an independent prognostic factor in NEC.
出处
《中国肺癌杂志》
CAS
2006年第1期55-59,共5页
Chinese Journal of Lung Cancer
基金
解放军总医院-军医进修学院"十.五"科研基金资助(01YM09)~~
