摘要
目的建立测定克拉霉素制剂含量的方法。方法利用克拉霉素与茜素红在水．醇介质中发生电荷转移反应，形成电荷转移络合物，采用可见分光光度法测定。结果荷移反应生成的荷移络舍物在526nm处有最大吸收，络合物的组成比是1：1，表观摩尔吸光系数是7．71×10^3L·mol^-1·cm^-1，药物浓度在5—90mg／L范围内服从比耳定律，方法平均回收率在98．0％以上，RSD为1．3％（n=6）。结论方法稳定、准确、灵敏、快速，对样品的测定结果令人满意。
OBJECTIVE To establish a method for rapid determination of clarithromycin. METHODS To utilize visible spectrophotometry based on the charge transfer reaction between clarithromycin as donor and alizarin red as acceptor in alcohol-water solution. RESULTS The apparent molar absorptivity of complex was 7.71 × 10^3 L · mol^-1 · cm^ -1 at 526nm. The composition was 1 ： 1. Beer~ law was obeyed in the range of 5 - 90mg/L of clarithromycin. The average recovery was over 98.0% . The average standard deviation of six determinations was 1.3% when the concentration of clarithromycin was 40mg/L. CONCLUSION The proposed method is stable, accurate, sensitive and rapid. It has been applied to the determination of clarithromycin in preparation with satisfactory results.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2006年第1期47-49,共3页
Chinese Journal of Modern Applied Pharmacy
关键词
电荷转移反应
茜素红
克拉霉素
分光光度法
charge transfer reaction
alizarin red, clarithromycin
spectrophotometry
