摘要
从犬细小病毒/犬瘟热进口二联苗中提取犬细小病毒基因组DNA,以此为模板进行PCR扩增,PCR产物经BamH I和XhoI双酶切后,克隆至pET22b(+)质粒的相应位点上,构建了原核表达载体pET22b/VP2.重组质粒经限制性内切酶酶切和核苷酸序列分析表明,VP2基因已正确克隆到pET22b(+)载体上,从而成功地构建了pET22b/VP2表达载体.
The genome DNA of canine parvovirus was extracted from CPV/CDV vaccine, which was used as templates for polymerase chain reaction （PCR） to amplify the VP2 gene. Prokaryotic expression vector of pET22b/VP2 was formed after PCR product was cloned into the restriction of plasmid pET22b（＋） by endonuclease BamHI and XhoI process. In this experiment, recombinant plasmid pET22b/VP2 was also identified by restriction enzymes analysis and nucleotidc sequence analysis. The results showed that the VP2 gene had been correctly cloned into plasmid pET22b（＋）.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2006年第1期60-62,共3页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
关键词
犬细小病毒
VP2基因
原核表达
载体构建
canine parvovirus
VP2 gene
prokaryotic expression vector
construction
