摘要
为建立决明子质量标准,采用高效液相法对决明子中主要成分大黄素进行定量分析,色谱柱Nova-pakC18(250 mm×4.6 mm,10μm),流动相乙腈-0.1%磷酸(80∶20),流速为1.0 ml/min,检测波长为254 nm。大黄素在0.1-0.5μg范围内呈良好的线性关系,回归方程为Y=653.95Х(r=0.9999),平均回收率为97.11%,RSD为1.28%,重现性RSD为1.89%。本方法简便、快速、灵敏、准确、重现性好,可作为决明子的质量控制指标。
Emodin from Semen Cassiae was determined by HPLC. The Nova-pakC18 column （250 mm ×4.6 mm 10μm） was used, the mobile phase was consisted of acetonitrile- 0.1%phosphoric acid （80 ： 20）, the flow rate was 1.0 ml/min, the UV detection wavelength was 254nm. The calibration curve is in good linearity over the range of 0.1 -0.5μg, and regression equation Y=653.95X （r=0. 9999）. The average recovery was 97.11%, RSD was 1.28%, RSD of reproduction was 1.89%. This method is simple, quick, sensitive, accurate, and has good reproducibility, and can be used to control the quality of Semen Cassiae.
出处
《福建中医学院学报》
2006年第1期40-41,共2页
Journal of Fujian College of Traditional Chinese Medicine
基金
福建省自然科学基金项目(C0410049)
