摘要
为了研究γ-干扰素(IFNγ)对大鼠胚胎基底前脑及隔区核团胆碱能神经元分化的作用,采用免疫组织化学方法对胆碱能神经元的特异性标记酶-胆碱乙酰基转移酶(ChAT)进行染色,ChAT阳性细胞的数量反映了胆碱能神经元的数量,并用14C-乙酰CoA作底物来检测ChAT活性。结果显示,IFNγ处理过的实验组,ChAT阳性细胞数量显著增加,ChAT活性也增加,这种增加被大鼠抗小鼠IFNγ单克隆抗体(Ab-IFNγ)完全拮抗。采用流式细胞术对细胞周期进行分析,细胞周期及细胞百分率无明显改变。用MAP2标记神经细胞,神经细胞数基本未增加。以上结果提示:IFNγ不能促进基底前脑和隔区神经元增殖,胆碱能神经元表达增加不是因为神经元数目增加而是分化的结果。
To investigate the effects of IFNγ on differentiation of cholinergic neurons in basal forebrain and septal nuclei （ BF/SN ）, immunocytochemistry was used to stain the special mark enzyme of cholinergic neurons-choline acetyhransferase （CHAT）. The number of ChAT positive （ ChAT＋） cells represent the number of cholinergic neurons. The 14 C-acetyl CoA was used as the substrate to assess the activity of CHAT. The results showed that the number of ChAT＋ cells significantly increased after IFNγ treatment,and the activity of ChAT also increased, which were antagonized by rat anti-mouse IFNγ monoclonal antibody（Ab-IFNγ） completely. The flow cytometry was used to analyze the cell cycle, the cell cycle and the cell percentage had no obvious change. No increase in the number of neurons marked by MAP2 was found. The results suggest that IFNγ can not promote the proliferation of the BF/SN neurons. The increase of cholinergic expression might be caused by differentiation instead of proliferation.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2006年第1期81-84,共4页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(No.39570249)资助项目
