摘要
目的探讨脑胶质瘤中hMLH1、hMSH2基因启动子区的甲基化状态,及其在肿瘤发生中的作用。方法采用甲基化特异性PCR(MSP)方法,检测42例脑胶质瘤hMLH1、hMSH2基因启动子区的甲基化状态。结果hMLH1启动子区甲基化2例(4.8%),hMSH2启动子区甲基化13例(31.0%),启动子区甲基化与性别、年龄、病理类型和病理分级无明显的相关性。结论hMSH2基因启动子的CpG岛在脑胶质瘤中有高甲基化现象,可能与胶质瘤的发生有关,而hMLH1基因启动子区甲基化少见。
Objective To explore the methylation status of hMLHland hMSH2 promoter region in glioma and its role in the oncogenesis. Methods Methylation status of the hMLH1 and hMSH2 promoter region was assayed in 42 glioma tissues by methylation-specific PCR （MSP）. Results Methylation in the hMLH1 and hMSH2 promoter regions was found in 2 ,cases （4.8%） and 13 （31.0%） respectively. Methylation status in the promoter region showed no obvious correlation with the sex, age, pathological type and grade. Conclusion hMSH2 promoter （CpG island） hypermethylation exists in glioma, which may correlates with the occurrence of glioma. However, methylation status of hMLH1 promoter is rare in giioma.
出处
《中国微侵袭神经外科杂志》
CAS
2006年第2期75-77,共3页
Chinese Journal of Minimally Invasive Neurosurgery
