摘要
从犬脑组织中提取总RNA,利用设计的一对引物N1/N2,扩增出1513bp的目的片段,通过SphI和SalI两个酶对扩增产物进行酶切,得到了457bp、833bp、233bp大小的3个预期片段,从而能在8 h内快速做出正确的诊断。试验表明,用该技术检测狂犬病病毒灵敏度高、特异性强,适合于实验室应用。
A new method by using PCR-RFLP for the rapid diagnosis of rabies was established, in which a 1513bp target fragment was amplified from brain tissues of dogs by PCR with a pair of the designed primers N1/N2, and then digested by SphI and Sal I, thus producing the predicted 3 fragments with 457 bp, 833 bp and 233 hp in size. These products were subjected to dectrophoresis on 1% agarose gel. By using the method to analyze the restriction fragment length polymorphism, a correct diagnosis for babies can be made within 8 hours. Owing to its higher sensitivity and specificity, this new method by using PCR-RFLP for rapid diagnosis of rabies is very suitable for practical use in laboratories.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2006年第2期168-169,共2页
Chinese Journal of Zoonoses
