摘要
目的:研究链脲佐菌素(STZ)诱导的糖尿病小鼠胰腺survivin基因mRNA表达,了解其在胰岛损伤中的作用。方法:小剂量多次注射STZ的方法建立糖尿病小鼠模型,每周测定体重及血糖,并采用实时荧光PCR方法检测胰腺survivin基因mRNA表达水平。结果:正常BALB/c小鼠胰腺有survivin基因表达。STZ组体重在4周内无明显改变;血糖在第1周即明显升高;survivin表达水平在3、4周显著升高。对照组体重持续增加,血糖及survivin表达水平各周间无显著差异。结论:正常小鼠胰腺有survivin基因表达,STZ注射后survivin表达水平显著升高,可能与胰岛恢复有关。
AIM： To investigate the expression of survivin in pancreas in the streptozotocin - induced diabetic mice. METHODS： Low dose of streptozotocin was used to induce diabetes mellitus in BALB/c mice. Body weight and blood glucose concentrations were examined at 1, 2, 3 and 4 weeks after the streptozotocin injection. Expression of survivin mRNA was detected by real- time FQ- PCR. RESULTS： Survivin was expressed in the pancreas of normal BALB/c mice. Low dose of streptozotocin provoked hyperglycaemia with increased survivin expression in the pancreas, but blood glucose concentration and expression of survivin was not significantly changed in control group. CONCLUSION： Survivin is expressed in the pancreas of normal BALB/c mice. Streptozotocin increases survivin expression in the pancreas, which may be related with islets regeneration.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第2期330-333,共4页
Chinese Journal of Pathophysiology
