摘要
目的观察遗传性视网膜色素变性rd小鼠视网膜外核层细胞层的病理和超微结构变化,为进一步应用rd小鼠进行实验研究提供数据。方法rd新生鼠42只,分别在第0、3、7、14、21、28、35d取眼球,立即经10%中性甲醛固定,光学显微镜下观察眼球水平位视网膜赤道部外核层细胞的厚度;另取眼球经2.5%戊二醛溶液固定,电镜观察。结果病理结果显示,rd小鼠视网膜外核层细胞层数或密度从第14d开始减少2.14层±0.60层,第21d减至近单层1.30层±0.37层,第35d减至0.57层±0.25层。电镜结果显示,rd小鼠视网膜光感受器细胞层生后第14d出现凋亡;外段盘膜部位线粒体从第7d开始出现部分溶解、减少,逐渐加重;外界膜也从第7d开始变为较连续,第21d后不连续。结论视网膜光感受器细胞外段的线粒体和外界膜对于视网膜盘膜的形成起重要作用。这一结果为进一步开展rd小鼠的研究提供了科学依据。
Objective To observe the variety of retinal outer nuclear layer cells in various growth duration in rd mice under the light microscopy and electron-microscopy. Methods Forty-two rd mice were sacrificed at different time namely postnatal day 1 and days 3,7,14,21,28,35. The eyeballs were immediately enucleated and fixed in 10% formaldehyde or 2. 5% glutaral solution. Routine pathologic procedure was performed and sections were stained by hematoxylin and eosin （HE）. The eyeball fixed with glutaral solution was examined by electron-microscopy. The layer number or density of retinal photoreceptor cells at the equator of the horizontal eyeball was evaluated under the light microscopy. Changes of the photoreceptor cells and the photoreceptor outer segment were observed by electron-microscopy. Results The layer number or density of retinal outer nuclear layer cells was gradually decreased from day 14（2. 14±0. 60）to day 36（0. 57±0. 25）. At day 21 ,almost one layer（ 1.30±0. 37）of outer nuclear layer cells was observed. The apoptosis of outer nuclear layer cells were seen on day 14 and the destruction of mitochondrion or disc membrane, discontinuous outer limiting membrane became more serious after day 7 upon examination by the electro-microscopy in rd mice. Conclusion Observation of retinal outer nuclear layer cells during the growth duration of 5 weeks in rd mice with light microscopy and electron-microscopy can offer an experimental approach for further study on rd mice.
出处
《眼科研究》
CSCD
北大核心
2006年第1期30-32,共3页
Chinese Ophthalmic Research
基金
广东省自然科学基金(010765)
广东省中医药管理局基金(10400640)资助
关键词
RD小鼠
光感受器细胞
发育
病理
电镜
rd mice
outer nuclear layer cells
development
light microscopy
electron-microscopy
