摘要
目的探讨兔角膜碱烧伤后血管内皮生长因子(vascular endothelial growth fac-tor,VEGF)的表达。方法用1mol·L-1氢氧化钠溶液烧伤兔角膜建立角膜碱烧伤动物模型,未烧伤眼作为对照;免疫组织化学方法检测正常对照组以及角膜碱烧伤后1d、4d、7d、10d、14d VEGF蛋白在角膜中的表达情况,同时用酶联免疫吸附测定法(enzymelinkedi mmunosorbent assay,ELISA)检测房水中VEGF蛋白的表达量。结果正常对照组中,VEGF蛋白在角膜上皮细胞和内皮细胞仅有弱阳性表达,基质层表达不明显;房水中VEGF表达量为(0.168±0.038)μg·L-1。碱烧伤后,角膜各层组织中VEGF蛋白表达较对照组增强(P<0.05),其中基质层可见大量炎性细胞浸润,伴随有VEGF蛋白在该区域的集中表达。房水中VEGF的表达量于烧伤后1d为(0.336±0.072)μg·L-1,随后持续升高,至14d达(1.294±0.216)μg·L-1,各检测点均较对照组有显著差异(P<0.05)。同时房水中VEGF表达量与角膜中检测结果呈正相关(P<0.01)。结论角膜碱烧伤后早期角膜组织和房水中VEGF蛋白的表达均显著增高,提示VEGF可能在损伤后的早期修复过程中发挥着作用。
Objective To investigate the expressions of vascular endothelial growth factor（VEGF） in the cornea and aqueous humor after alkali burn of cornea in rabbit. Methods Alkali burn of cornea in rabbit was induced by application of 1 mol·L^-1 NaOH in one eye, the other was served as normal control. Expression of VEGF in the cornea was detected by immunohistochemistry at the 1st day, 4th day, 7th day, 10th day and 14th day respectively. VEGF in aqueous humor was quantified by ELISA assays. Results In the normal control, weak expression of VEGF was presented in the epithelium and endothelitun of the cornea, but no obvious expression was observed in the stroma. VEGF concentration in the aqueous humor was（0. 168±0. 038） μg·L^-1. After alkali burn, the expression of VEGF in the cornea was stronger than that of the normal control（P 〈0.05）. A large amount of infiltration inflammatory cells were found in the stroma, accompanying by the expression of VEGF in that area. In the aqueous humor,VEGF level was （0. 336±0. 072） μg·L^-1 at Ist day and increased to （1. 294±0. 216）μg·L^-1 at 14th day, which increased significantly compared with the control（P〈0.05）. Expression of VEGF in the aqueous humor was positively correlated with that in the cornea（P〈0.01 ）. Conclusion Expressions of VEGF in the cornea and the aqueous humor were significantly increased in the early stage after alkali burn of rabbit cornea, which indicated that VEGF may play a role in the early healing process of cornea injury.
出处
《眼科新进展》
CAS
2006年第2期103-106,共4页
Recent Advances in Ophthalmology
关键词
角膜
房水
碱烧伤
血管内皮生长因子
cornea
aqueous humor
alkali burn
vascular endothelial growth factor
